RESUMO: Objetivo: traduzir o instrumento em língua francesa "Questionnaire D'Alimentation" para o português brasileiro e realizar a adaptação transcultural em adolescentes brasileiros. Métodos: a tradução foi realizada com base em protocolo que consistiu na tradução para o português brasileiro (por um professor formado em Letras e uma Cirurgiã Dentista fluentes em francês), tradução reversa para o francês (realizada por dois professores de francês), revisão por Comitê de especialistas (duas tradutoras, uma nativa e uma juramentada, e duas professoras universitárias, sendo uma Cirurgiã Dentista e uma Fonoaudióloga) e equivalência cultural (pré-teste). A versão utilizada no pré- teste consistiu de 26 questões com cinco respostas possíveis (5-Likert), distribuídas em cinco domínios (Alimentação- mastigação, Hábitos, Carnes, Frutas e Legumes). O pré-teste e teste-reteste foram realizados com uma amostra de 20 adolescentes (10 meninos/10 meninas) de escolas públicas de Piracicaba (Brasil). Nesta fase, a alternativa "não entendi" foi incluída a cada questão, a fim de identificar aquelas não compreendidas. A confiabilidade teste-reteste foi avaliada para cada domínio utilizando os coeficientes de correlação intra-classe (CCI). Resultados: no pré-teste, foi observada uma excelente compreensão do instrumento; no teste-reteste, os CCIs variaram entre 0,45-0,81 (moderada a excelente concordância). Conclusão: a versão em Português do Questionnaire D'Alimentationmostrou ser de fácil compreensão por parte dos adolescentes brasileiros e útil na avaliação da função mastigatória e dos distúrbios de alimentação e deglutição que podem alterar a ingestão de alimentos.

ABSTRACT: Purpose: to translate the French language instrument "Questionnaire D'Alimentation" to Brazilian Portuguese and culturally adapt it for adolescents. Methods: the translation was based on a protocol that consisted of translation to Brazilian Portuguese (by a professor with a Literary Arts degree and a Doctor of Dental Surgery, both fluent in French and Brazilian Portuguese); back-translation into French; revision by a Committee of specialists (two translators, one native and one sworn translator, and two university professors, one being a Doctor of Dental Surgery and a Speech Therapist) and cultural equivalence (pre-test). The version used in the pre-test consisted of 26 questions with five possible responses (5-Likert), distributed in five domains (Food-Mastication, Habits, Meats, Fruits and Vegetables). The pre-test and test-retest was performed with a sample of 20 adolescents (10 boys/10 girls) from public schools of Piracicaba (Brazil). At this stage, the alternative "I did not understand" was added to each question in order to identify those that were not understood. Test-retest reliability was assessed for each domain using intra-class correlation coefficients (ICCs). Results: in the pre-test, an excellent comprehension of the instrument was observed; in test-retest, ICCs ranged from 0.45 to 0.81 (moderate to excellent agreement). Conclusion: the Portuguese version of the Questionnaire D'Alimentationhas shown to be easy to understand by Brazilian adolescents and useful in the evaluation of the masticatory function and feeding or swallowing disorders that may affect food intake.

Introdução Um dos principais problemas dos serviços públicos de saúde, em que a equipe de saúde bucal da família está inserida, é o acesso dos usuários ao tratamento odontológico na atenção primária, principalmente no que se refere à doença cárie. Objetivo O objetivo deste estudo foi avaliar a relação entre risco familiar – para priorização de visitas domiciliares – e condições de saúde bucal, visando a prover evidência sobre o primeiro indicador para organização da demanda em saúde bucal, na Estratégia Saúde da Família (ESF). Método A verificação do risco familiar baseou-se na ficha A do SIAB, utilizada para o cadastramento das famílias na ESF. Onze cirurgiões dentistas examinaram as condições de saúde bucal de 1165 pessoas (608 de 12 a 19 anos e 557 de 35 a 44 anos de idade), classificando-as em seis códigos de A a F. Empregou-se a regressão logística multinomial (α=0,05) para análise da associação entre as variáveis de risco familiar e a situação de saúde bucal. Resultado Houve associação significativa entre risco familiar e presença da doença cárie com necessidade de tratamento (OR: 2,08; p<0,0001). Conclusão As pessoas que possuem risco familiar teriam duas vezes mais chance de apresentar a doença cárie em comparação às sem risco, corroborando a relevância deste elemento na organização da demanda em saúde bucal.

Introduction One of the main problems of the public health services, in which the family oral health team is included, is access by users to dental treatment in primary care, with particular reference to caries disease. Objective The aim of this study was to evaluate the relationship between family risk, for prioritization of home visits and oral health conditions, with a view to providing evidence about the first indicator for organizing the demand for oral health in the Family Health Strategy (FHS). Method The application of family health is based on Form A of the primary care information database SIAB ("Sistema de Informação de Atenção Básica"), used for registering families with the FHS. Eleven dentists examined the oral health conditions of 1165 persons (608 from 12 to 19 years; and 557 from 35 to 44 years of age), classifying them into six codes from A to F. Multinomial logistic regression was used (α=0.05) to analyze the association between family risk variables and oral health situation. Result There was significant association between family risk and presence of caries disease with treatment needs (OR: 2.08, p<0.0001). Conclusion Persons who have family risk would have twice as much chance of presenting caries disease in comparison with those without risk, corroborating the relevance of this element in organizing the demand for oral health.

OBJECTIVE: To investigate the most commonly fabricated orthodontic retainers. METHODS: Information on the type and amount of maxillary and mandibular retainers produced in a three-month period was collected from six laboratories in the cities of São Paulo, Mauá and Guarulhos - Brazil. The retainers were grouped according to the total production. For the maxillary arch, the groups were: 1S - Begg retainer, 2S - Hawley retainer, 3S - transpalatal arch retainer, 4S - buccal resin-arch retainer and 5S - vacuum-formed retainer, Planas appliance, bonded lingual retainer and V-loop bonded lingual retainer. The groups relative to the mandibular arch were: 1I - 3-3 bonded lingual retainer (canine to canine), 2I - Hawley retainer and V-loop bonded lingual retainer, 3I - Begg retainer, 4I - buccal resin-arch retainer, vacuum-formed retainer and Planas appliance. The data were presented in box plots. Groups were compared using the Student's-t test with Bonferroni correction. RESULTS: The average of maxillary appliances fabricated ranged from 189.5 (1S) to 3.95 (5S). There were significant differences between groups 1S versus 5S and 2S versus 5S (p < 0.0001). Mean values for the mandibular retainers ranged from 55.3 (1I) to 4.2 (4I). Significant difference was observed between groups 2I and 4I (p < 0.0001). CONCLUSIONS: For the maxillary arch, the most requested retainers were Begg and Hawley retainers. Regarding the mandibular arch, bonded lingual retainers and Hawley retainer were the most frequent ones.

OBJECTIVE: Assess the association between nonnutritive sucking habits and anterior open bite in the deciduous dentition of Japanese-Brazilian children. METHODS: 410 children of Japanese origin were assessed, 206 boys and 204 girls, between 2 and 6 years of age, in schools in São Paulo State, Brazil. Questionnaires concerning their nonnutritive sucking habits were sent to their legal guardians. Chi-square tests (p<0.05) were applied to assess the association between nonnutritive sucking habits and anterior open bite, and the logistic regression test to obtain the relative risk. RESULTS: The prevalence of sucking habits found in the sample was of 44.6% and for the anterior open bite, 4.4%. There was a statistically significant association between anterior open bite and sucking habits (O.R.=10.77), persistence of sucking habits from 2 to 4 years old (O.R.=22.06), and the persistence of sucking habits from 4 to 6 years old (O.R.=17.31). As for the interruption period of the habit, the group that had interrupted the habit for a period equal or inferior to six months showed an increased prevalence of open bite compared to the group without this habit or in which the habit was interrupted for more than six months. CONCLUSION: Japanese-Brazilian children that had sucking habits have greater chance of acquiring anterior open bite in the deciduous dentition.

OBJETIVO: avaliar comparativamente a prevalência das características de espaçamento anterior em nipo-brasileiros e leucodermas, na dentição decídua. MÉTODOS: duas amostras de crianças com 2 a 6 anos de idade foram selecionadas: 405 nipo-brasileiros de 36 escolas do estado de São Paulo e 510 leucodermas de 11 instituições públicas da cidade de São Paulo. As variantes oclusais foram classificadas em quatro categorias, nas arcadas superior e inferior: espaçamento generalizado; somente espaços primatas; ausência de espaços; e apinhamento. Foi aplicada regressão logística para análise do efeito dos fatores idade, sexo e grupo étnico sobre a prevalência das características oclusais (α = 0,05). RESULTADOS: em nipo-brasileiros, o espaçamento generalizado foi a característica mais prevalente nas arcadas superior (46,2%) e inferior (53,3%). A frequência dos espaços primatas foi maior na arcada superior (28,2% versus 15,3%). Para a ausência de espaços (21,7-26,4%) e o apinhamento (4,0-4,9%), a variação entre as arcadas foi relativamente pequena. Em leucodermas, a ausência de espaços e a presença exclusiva dos primatas evidenciaram distribuição similar à observada nos nipo-brasileiros. O espaçamento generalizado foi diagnosticado em aproximadamente 50% das arcadas. A prevalência de apinhamento foi maior na arcada inferior (12,8% versus 3,9%). O único modelo de regressão logística que apresentou algum fator significativo foi o ajustado para a prevalência de apinhamento. Apenas o fator racial foi significativo (p < 0,001). CONCLUSÃO: sugere-se que as características de espaçamento anterior, na dentição decídua, não estariam condicionadas à idade ou ao sexo. Contudo, leucodermas teriam chances 2,8 vezes maiores de apresentar apinhamento na arcada inferior, em comparação aos nipo-brasileiros.

OBJECTIVE: To carry out a comparative evaluation of the prevalence of anterior spacing characteristics in Japanese-Brazilian and white children in primary dentition. METHODS: Two selected samples of children aged 2-6 years were: 405 Japanese-Brazilians from 36 schools in the State of São Paulo and 510 white children from 11 public institutions in São Paulo city. The spacing features in the maxillary and mandibular arches were assigned to four categories: generalized spacing; only primate spaces; no spacing; and crowding. Logistic regression was used to analyze the effect of age, gender and ethnic group on the prevalence of the occlusal characteristics (α=0.05). RESULTS: In Japanese-Brazilians, generalized spacing was the most prevalent trait in the maxillary (46.2%) and mandibular (53.3%) arches. The frequency of primate spaces was higher in the maxillary arch (28.2% versus 15.3%). Concerning no spacing (21.7-26.4%) and crowding (4.0-4.9%), the variation between arches was relatively small. In white children, no spacing and exclusive presence of primate spaces showed distributions similar to that observed in Japanese-Brazilians. Generalized spacing was diagnosed in approximately 50% of the arches. Crowding prevalence was higher in the mandibular arch (12.8% versus 3.9%). The regression model adjusted for crowding prevalence was the only significant one. Only racial factor was significant (p<0.001). CONCLUSION: It may be suggested that anterior spacing features in primary dentition would not be influenced by age or gender. Nevertheless, white children would have 2.8 times more chances of presenting crowding in the mandibular arch, in comparison with Japanese-Brazilians.

To achieve proper recontouring of anterior and posterior teeth, to obtain optimal morphology during enamel stripping, it is important to be aware of dental anatomy. This study aimed at evaluating crown dimensions and proximal enamel thickness in a sample of 40 extracted sound, human, mandibular, second bicuspids (20 right and 20 left). Mesiodistal, cervico-occlusal and buccolingual crown dimensions were measured using a digital caliper, accurate to 0.01 mm. Teeth were embedded in acrylic resin and cut along their long axes through the proximal surfaces to obtain 0.7 mm-thick central sections. Enamel thickness on the cut sections was measured using a perfilometer. Comparative analyses were carried out using the Student's-t test (α= 5%). The mean mesiodistal crown widths for right and left teeth were 7.79 mm (± 0.47) and 7.70 mm (± 0.51), respectively. Mean cervico-occlusal heights ranged from 8.31 mm (± 0.75) on the right to 8.38 mm (± 0.85) on the left teeth. The mean values for the buccolingual dimension were 8.67 mm (± 0.70) on the right and 8.65 mm (± 0.54) on the left teeth. The mean enamel thickness on the mesial surfaces ranged from 1.35 mm (± 0.22) to 1.40 mm (± 0.17), on the left and right sides, respectively. On the distal surfaces, the corresponding values were 1.44 mm (± 0.21) and 1.46 mm (± 0.12). No significant differences were found between measurements for right and left teeth. However, enamel thickness was significantly greater on the distal surfaces, compared with the mesial surfaces.

OBJECTIVE: Nutritional, immunological and psychological benefts of exclusive breastfeeding for the frst 6 months of life are unequivocally recognized. However, mothers should also be aware of the importance of breastfeeding for promoting adequate oral development. This study evaluated the association between breastfeeding and non-nutritive sucking patterns and the prevalence of anterior open bite in primary dentition. MATERIAL AND METHODS: Infant feeding and non-nutritive sucking were investigated in a 3-6 year-old sample of 1,377 children, from São Paulo city, Brazil. Children were grouped according to breastfeeding duration: G1 - non-breastfed, G2 - shorter than 6 months, G3 - interruption between 6 and 12 months, and G4 - longer than 12 months. Three calibrated dentists performed clinical examinations and classifed overbite into 3 categories: normal, anterior open bite and deep bite. Chi-square tests (p<0.05) with odds ratio (OR) calculation were used for intergroup comparisons. The impact of breastfeeding and non-nutritive sucking on the prevalence of anterior open bite was analyzed using binary logistic regression. RESULTS: The prevalence estimates of anterior open bite were: 31.9% (G1), 26.1% (G2), 22.1% (G3), and 6.2% (G4). G1 would have signifcantly more chances of having anterior open bite compared with G4; in the total sample (OR=7.1) and in the subgroup without history of non-nutritive sucking (OR=9.3). Prolonging breastfeeding for 12 months was associated with a 3.7 times lower chance of having anterior open bite. In each year of persistence with non-nutritive sucking habits, the chance of developing this malocclusion increased in 2.38 times. CONCLUSIONS: Breastfeeding and non-nutritive sucking durations demonstrated opposite effects on the prediction of anterior open bite. Non-breastfed children presented signifcantly greater chances of having anterior open bite compared with those who were breastfed for periods longer than 12 months, demonstrating the benefcial infuence of breastfeeding on dental occlusion.

This study aimed at evaluating measurements relative to the mesiodistal crown width and enamel thickness of maxillary first bicuspids. The sample consisted of 40 extracted sound bicuspids (20 right and 20 left), selected from white patients (mean age: 23.7 ± 4.2 years), who were treated orthodontically with tooth extraction at a private clinic in São Paulo, SP, Brazil. All teeth were embedded in acrylic resin and cut along their long axis through the proximal surfaces, parallel to the buccal side, to obtain 0.6-mm central sections. The mesiodistal crown width and proximal enamel thickness were measured using a stereoscopic microscope connected to a computer. Measurements for right and left teeth, as well as the mesial and distal enamel thicknesses in the total sample, were compared by the Wilcoxon test (α = 0.05). The mesiodistal crown width mean values found were 7.51 mm (± 0.54) on the right side and 7.53 mm (± 0.35) on the left side. The mean enamel thickness on the distal surfaces for both sides was 1.29 mm (right: s.d. = 0.12 and left: s.d. = 0.18). The mean values for the mesial surfaces were 1.08 mm (± 0.14) and 1.19 mm (± 0.25), on the right and the left sides, respectively. No significant differences were found between the crown measurements and enamel thicknesses on the left and right sides. However, enamel thickness was significantly greater on the distal surfaces. Reliable measurements of enamel thickness are useful to guide stripping, which may be an attractive alternative to tooth extraction because it allows the transverse arch dimension to be maintained.

This study evaluated the effect of breast- and bottle-feeding duration on the age of pacifier use persistence. Questionnaires (n = 723) with information on nutritive and nonnutritive sucking habits of children aged 3-6 years were assessed. The sample was divided according to breastfeeding duration: G1 - non-breastfed, G2 - up to 3 months, G3 - discontinued between 4 and 6 months, G4 - discontinued between 7 and 12 months, and G5 - longer than 12 months. The children were also assigned to 4 groups by age of pacifier use persistence, as well as by age of bottle-feeding persistence: no habits, up to 2 years, 3-4 years and 5-6 years. Associations between nutritive sucking habits and pacifier use were analyzed using logistic regression. The larger breastfeeding groups were G2 (37.9%) and G4 (19.4%). Many children discontinued pacifier use and bottle-feeding at 3-4 years of age (24.9% and 40.1%, respectively). Chances of non-breastfed children (G1) with prolonged pacifier-sucking habits, in the three age ranges, were progressively higher in comparison with group G4 (OR: 4.0-7.5, p < 0.01). When comparing bottle-fed with non bottle-fed children, the age range at which bottle-feeding had been discontinued was significantly associated with that of pacifier use cessation: up to 2 years (OR = 6.2), 3-4 years (OR = 7.6) and 5-6 years (OR = 27.0), p < 0.01. It may be suggested that breastfeeding duration has an inversely proportional effect on the age of pacifier use persistence. Bottle-fed children who use pacifiers tend to discontinue these habits at the same period.

The aim of this study was to comparatively assess dental arch width, in the canine and molar regions, by means of direct measurements from plaster models, photocopies and digitized images of the models. The sample consisted of 130 pairs of plaster models, photocopies and digitized images of the models of white patients (n = 65), both genders, with Class I and Class II Division 1 malocclusions, treated by standard Edgewise mechanics and extraction of the four first premolars. Maxillary and mandibular intercanine and intermolar widths were measured by a calibrated examiner, prior to and after orthodontic treatment, using the three modes of reproduction of the dental arches. Dispersion of the data relative to pre- and posttreatment intra-arch linear measurements (mm) was represented as box plots. The three measuring methods were compared by one-way ANOVA for repeated measurements (α = 0.05). Initial / final mean values varied as follows: 33.94 to 34.29 mm / 34.49 to 34.66 mm (maxillary intercanine width); 26.23 to 26.26 mm / 26.77 to 26.84 mm (mandibular intercanine width); 49.55 to 49.66 mm / 47.28 to 47.45 mm (maxillary intermolar width) and 43.28 to 43.41 mm / 40.29 to 40.46 mm (mandibular intermolar width). There were no statistically significant differences between mean dental arch widths estimated by the three studied methods, prior to and after orthodontic treatment. It may be concluded that photocopies and digitized images of the plaster models provided reliable reproductions of the dental arches for obtaining transversal intra-arch measurements.

This study aimed at investigating the effect of the partial erasing of DenOptix® system storage phosphor plates on the image quality of digital radiographs. Standardized digital radiographs were acquired of a phantom mandible, using size 2 intraoral DenOptix® storage phosphor plates (n = 10). Subsequently, the active areas of the plates were placed in a viewing box with a constant light intensity of 1,700 lux for 130 seconds to achieve complete erasing (control plate), as well as for 0, 5, 10, 15, 20, 25, 34, 66, and 98 seconds, to compose the experimental group of partially erased plates. The same exposure settings were repeated using the control and experimental plates, which were scanned at a resolution of 300 dpi. Five radiologists independently examined the pairs of digital radiographs obtained with the control and partially erased plates, in random order, and indicated the best image for oral diagnosis. Cochran-Mantel-Haenszel’s chi-square test, at a significance level of 5%, was used to compare the percentages of superior quality images in each combination of control and partially erased plates, subjectively assessed. No significant differences were found between radiographic images acquired with control and partially erased plates, except for the combination of 0 second (30%) versus 130 seconds (70%), p = 0.0047. It can be concluded that, under adequate light intensity conditions, erasing intraoral DenOptix® storage phosphor plates may require time intervals of as little as 5 seconds.

The aim of this study was to investigate a model of inducing enamel subsurface demineralization for evaluating diagnostic imaging methods. Fifty sound teeth had their crowns coated with acid-resistant varnish, leaving a 7 mm² circular window of exposed enamel in one of the proximal surfaces. The specimens were assigned to 5 groups (4 experimental and the control) and were kept immersed in a buffer solution at pH 4.8, 50% saturated in relation to enamel, for 60, 75, 90 and 120 days, or were not exposed to the solution. Digital radiographs were taken, prior to and after each immersion period, and interpreted by a radiologist. For validation, the enamel was submitted to microhardness profiling. In addition, the solutions were analyzed for phosphorus (Pi) and calcium (Ca) concentrations. Microhardness data were evaluated using two-way ANOVA (p<0.05) and Tukey's test. Pi and Ca concentrations were compared using one-way ANOVA (alpha = 0.05). The performance of radiography was assessed by ROC analysis. The microhardness values for the control group ranged from 405 to 432 KHN. Of the 4 experimental groups, the 60-day group demonstrated the highest values (179-379 KHN) and the 120-day group, the lowest values (103-277 KHN). The lowest total concentrations of Pi and Ca (1.74 mM and 2.63 mM, respectively) were found in the solutions of the 60-day group, indicating the lowest rate of mineral loss, however, there was no significant difference among the other experimental groups. The performance for detecting images consistent with caries lesions was high (Az = 0.89). Therefore, this in vitro model of inducing demineralization may be considered suitable for research intended to evaluate imaging methods for the diagnosis of proximal caries lesions.

O objetivo deste estudo foi avaliar prevalência de mordida cruzada posterior em crianças com hábito de sucção de chupeta que persistiu até diferentes idades. Crianças dos 3 aos 6 anos de idade foram aleatoriamente selecionadas de pré-escolas públicas em São Paulo, SP, Brasil. Solicitou-se às mães que respondessem a um questionário sobre hábitos de sucção não-nutritivos. A amostra consistiu de 366 crianças distribuídas em 2 grupos: controle (n = 96) e usuários de chupeta (n = 270). Os usuários de chupeta foram, então, separados em 3 subgrupos de acordo com a idade de persistência do hábito: P1 - até 2 anos de idade; P2 - entre 2 e 4 anos de idade e P3 - entre 4 e 6 anos de idade. Um cirurgião-dentista avaliou a oclusão das crianças por exame clínico. As associações entre intervalo de interrupção do hábito e prevalência de mordida cruzada posterior foram analisadas pelo teste Qui-Quadrado (p < 0,05). A prevalência de mordida cruzada posterior foi significativamente mais elevada em usuários de chupeta (20,4%), em comparação às crianças controles (5,2%), p < 0,01. A mordida cruzada posterior unilateral foi mais prevalente do que a bilateral em usuários de chupeta (9,8% versus 3,6%). As mordidas cruzadas posteriores funcionais foram diagnosticadas em 3,1% das crianças controles e 7% dos usuários de chupeta. As freqüências de mordida cruzada posterior foram notavelmente elevadas nos 3 subgrupos de usuários de chupeta, P1, P2 e P3, correspondendo a 17,2%, 16,9% e 27,3%, respectivamente. A alta prevalência de mordida cruzada posterior pode estar associada com hábitos de sucção de chupeta que persistiram além dos 2 anos de idade.

The aim of this study was to evaluate the prevalence of posterior crossbite among children whose pacifier-sucking habit persisted until different ages. Children aged 3 to 6 years were randomly selected from public preschools in São Paulo, SP, Brazil. Their mothers were asked to complete a questionnaire on non-nutritive sucking behaviors. The sample consisted of 366 children assigned to 2 groups: control (n = 96) and pacifier users (n = 270). Pacifier users were further assigned to 3 subgroups, according to the age of habit persistence: P1 - until 2 years of age; P2 - between 2 and 4 years of age; and P3 - between 4 and 6 years of age. One dentist assessed the children for occlusal relationships through clinical examination. Associations between the age interval of habit discontinuation and the prevalence of posterior crossbite were analyzed using the chi-square test (p < 0.05). The prevalence of posterior crossbite was significantly higher among pacifier users (20.4%), compared to control children (5.2%), p < 0.01. Unilateral posterior crossbite was more prevalent than bilateral crossbite among pacifier users (9.8% versus 3.6%). Functional posterior crossbites were diagnosed in 3.1% of the control children and 7% of the pacifier users. The frequencies of posterior crossbite were notably high for children in the 3 pacifier subgroups, P1, P2, and P3, corresponding to 17.2%, 16.9%, and 27.3%, respectively. The high prevalence of posterior crossbite may be associated with pacifier-sucking habits that persisted after 2 years of age.

O objetivo deste estudo foi avaliar o efeito da irradiação por baixa dose de elétrons sobre características morfológicas da reparação tecidual na pele de rato. Foram realizadas feridas cirúrgicas com um gabarito de 2,3 x 1,4 cm em 84 ratos machos, divididos em 4 grupos: IG1, irradiado imediatamente; IG2, irradiado 3 dias após a produção da ferida; CG1 e CG2, grupos controles. Os ratos dos grupos IG1 e IG2 tiveram suas feridas expostas a 1 Gy de radiação por elétrons com 6 MeV, imediatamente após a cirurgia e ao terceiro dia pós-operatório, respectivamente. Procedeu-se às avaliações qualitativas e histofotométrica das estruturas de reparação tecidual. Os dados foram submetidos a ANOVA e teste Tukey (a = 0,05) e análise de regressão. O processo de reparação apresentou-se atrasado desde o primeiro tempo de sacrifício em ambos os grupos irradiados, mas para IG1, a reparação tecidual foi similar a CG1. Por outro lado, para IG2, o retardo foi mais pronunciado. Com base nas avaliações histológicas, é possível concluir que uma baixa dose de radiação por elétrons retardou a reparação na pele de rato. O atraso foi maior na pele irradiada 3 dias após o início da reparação tecidual. No entanto, esta irradiação por elétrons de baixa energia não impediu a cicatrização.

The aim of this study was to assess the effect of low-dose electron irradiation on morphological features of the wound healing process in rat skin. Surgical wounds were inflicted with a 2.3 x 1.4 cm template on 84 male rats that were assigned to 4 groups: IG1, immediately irradiated; IG2, irradiated 3 days after inflicting the wound; CG1 and CG2, control groups. Rats in IG1 and IG2 groups had their wounds exposed to 1 Gy of 6 MeV electron beam radiation, immediately after surgery and on the third postoperative day, respectively. Qualitative and histophotometric evaluations of tissue repair structures were carried out. Data were analyzed by ANOVA and Tukey's test (alpha = 0.05) and regression analysis. The repair process was delayed since the first sacrifice time in both irradiated groups, but in IG1, wound healing was closer to that of CG1; whereas in IG2, the delay was more pronounced. Based on the histological findings, it is possible to conclude that a low-dose of electron radiation delayed tissue repair in rat skin. The delay was longer in the skin irradiated 3 days after the beginning of tissue repair. However, the low-energy electron irradiation did not prevent wound healing.

O objetivo desta pesquisa experimental foi investigar a acurácia da detecção de desmineralizações em esmalte por meio de radiografias convencionais, digitais e digitalizadas, e compará-las às imagens por subtração logarítmica. Foram induzidas desmineralizações subsuperficiais no esmalte de uma das faces proximais de 49 dentes terceiros molares hígidos. Radiografias padronizadas dos dentes foram obtidas antes e após a fase de desmineralização com o emprego de três sistemas digitais - CygnusRay MPS®, DenOptix® e DIGORA® - bem como do filme InSight®. Três radiologistas interpretaram os pares de radiografias convencionais, digitais e digitalizadas, em duas ocasiões diferentes. As imagens por subtração logarítmica foram avaliadas por um quarto radiologista apenas uma vez. O diagnóstico radiográfico foi validado pela mensuração da microdureza do esmalte seccionado longitudinalmente, nas áreas-teste das faces proximais. A acurácia foi estimada pela análise "Receiver Operating Characteristic" (ROC). Aplicou-se o teste Qui-Quadrado, em um nível de significância de 5%, para comparar as áreas sob as curvas ROC (Az) das diferentes modalidades de imagens radiográficas. Em se tratando das radiografias, o sistema DenOptix® (Az = 0,91) e as radiografias convencionais (Az = 0,90) apresentaram os maiores valores de acurácia em relação às outras três modalidades de imagem. No entanto, as imagens por subtração logarítmica (Az = 0,98) superaram as radiografias convencionais, digitais e digitalizadas (p = 0,0000). Conclui-se que o sistema DenOptix® e as radiografias convencionais demonstram melhor desempenho no diagnóstico das desmineralizações subsuperficiais em esmalte. A subtração logarítmica aprimora significativamente a detecção radiográfica.

This experimental research aimed at evaluating the accuracy of enamel demineralization detection using conventional, digital, and digitized radiographs, as well as to compare radiographs and logarithmically contrast-enhanced subtraction images. Enamel subsurface demineralization was induced on one of the approximal surfaces of 49 sound third molars. Standardized radiographs of the teeth were taken prior to and after the demineralization phase with three digital systems - CygnusRay MPS®, DenOptix® and DIGORA® - and InSight® film. Three radiologists interpreted the pairs of conventional, digital, and digitized radiographs in two different occasions. Logarithmically contrast-enhanced subtraction images were examined by a fourth radiologist only once. Radiographic diagnosis was validated by cross-sectional microhardness profiling in the test areas of the approximal surfaces. Accuracy was estimated by Receiver Operating Characteristic (ROC) analysis. Chi-square test, at a significance level of 5%, was used to compare the areas under the ROC curves (Az) calculated for the different imaging modalities. Concerning the radiographs, the DenOptix® system (Az = 0.91) and conventional radiographs (Az = 0.90) presented the highest accuracy values compared with the other three radiographic modalities. However, logarithmically contrast-enhanced subtraction images (Az = 0.98) were significantly more accurate than conventional, digital, and digitized radiographs (p = 0.0000). It can be concluded that the DenOptix® system and conventional radiographs provide better performance for diagnosing enamel subsurface demineralization. Logarithmic subtraction significantly improves radiographic detection.